Abstract
- This updated edition explores different spectroscopic and biophysical methods used to study the structure and folding of RNA and to follow their interactions with proteins. The book expands into protocols for dynamic and single-molecule analyses, addressing the new challenges that have emerged since the discovery of novel RNAs and their interaction networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.